Single-cell transcriptomics of the midbrain of IPD patients and healthy controls
Transcriptomic studies in postmortem human midbrain so far were based on bulk RNA-seq technologies, which lack the necessary resolution to study the contribution of individual cell types to disease pathology. To overcome these challenges, we performed single-cell RNA sequencing of postmortem adult human midbrain tissue of five IPD patients and six age-matched healthy controls to obtain an unbiased and global view of the cell-type composition and cellular phenotype of IPD. We identified all the major cell-types in the midbrain including different neuronal types, glia cells, pericytes, endothelial and ependymal cells and we found the differentially expressed genes in each of the cell type between healthy control and IPD patients. In addition, we report a disease-specific upregulation of microglia and astrocytes further strengthening the role of neuroinflammation in Parkinson’s disease

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